Sequential reading of a stepwise-shortened peptide immobilized on nanopore

Nature, Published online: 29 July 2026; doi:10.1038/s41586-026-10881-1A nanopore-based ‘chop and measure’ method sequences peptides at single-amino-acid resolution by using enzymatic digestion to progressively shorten the N terminus one residue at a time, together with repetitive N-terminus re-reading.